This is a two-day course, that builds on the introductory course and will provide students with the knowledge required to:
- set up and use Phase contrast, Interference contrast and Polarising microscopes
- select appropriate dyes and filter sets for Fluorescent microscopy
- choose appropriate Fluorescent imaging modalities
- be critical of data and troubleshoot their imaging.
Topics covered include, but not limited to:
- Components and light paths in Phase contrast, Interference contrast and Polarising microscopes, including reflected light techniques
- Microscope Beam Paths and Components in fluorescent microscopy
- Properties of Fluorescence molecules and Fluorophore selection
- The emission equation
- Properties of filters and the filter cube
- Matching filters and fluorochromes
- Fluorescent antibody and protein experimental design considerations
- Principles, characteristics and the pros and cons of Widefield Fluorescence, Deconvolution Microscopy Structured illumination (Apotome), Confocal and Multiphoton Microscopy (When to use what!)
- Identifying problems and finding solutions
- Resolution and Signal to Noise issues
- Optical aberrations: what are they, what do they do to our image and how do we fix it?
- Introduction to the principles behind Spectral unmixing,TIRF, Super Resolution, STED, PALM, STORM, SIM GSD, and their relatives
- Non-imaging fluorescence techniques: FCS, FLIM.
Cost: AUD$250.00 per person (GST not included)