First Cryo-EM suite in the ACT

Friday 15 October 2021
An electron microscope sits on the floor of a room, to the right of a table that has two computer monitors with a keyboard and other controls on it. There is an armless office chair in front of the table.
ZEISS Crossbeam 550 FIB-SEM

In the last 12 months, the Centre for Advanced Microscopy (CAM), ANU, has established an NCRIS-supported cryo-EM suite featuring two high-end transmission electron microscopes for screening and automated data analysis of frozen samples for single particle analysis, MicroED and tomography (JEOL JEMF200 and JEOL 200kV CryoARM) and a ZEISS Crossbeam 550 FIB-SEM. The latter comprises a Leica cryo stage and transfer system which facilitates cryo block face imaging, as well as the production of on-grid and lift-out TEM lamella for viewing in the JEOL 200kV CryoARM. 

For a correlative workflow, additional upgrades include a new Linkham Scientific cryo stage (CMS196) for the ZEISS LSM800 with Airyscan and ZEISS Atlas 5 and ZEN Connect software, which facilitate an enhanced workflow for correlative cryo-light microscopy and tomography/3D cryo volume imaging.

Please contact CAM if you have any questions regarding these instruments.

Updated:  30 November 2021/Responsible Officer:  Director CAM/Page Contact:  CAM Web Admin