One big step further to a full cryo suite at ANU

Tuesday 20 October 2020
An electron microscope sits on the floor of a room, to the right of a table that has two computer monitors with a keyboard and other controls on it. There is an armless office chair in front of the table.
ZEISS Crossbeam 550 FIB-SEM

ANU installs first NCRIS supported instrument: Zeiss Crossbeam 550 FIB/FESEM

CAM recently welcomed the installation of the ZEISS Crossbeam 550 FIB-SEM on its path to develop a fully equipped correlative cryo platform. The Crossbeam comprises a Leica cryo stage and transfer system which will facilitate cryo block face imaging, as well as the production of on-grid TEM lamella for viewing in the JEOL 200kV CryoARM (NCRIS co-funded; expected March 2021).

For a correlative workflow, additional upgrades include a new Linkam Scientific cryo stage (CMS196) for the ZEISS LSM800 with Airyscan and the ZEISS Atlas 5 and ZEN Connect software, which will facilitate an enhanced workflow for correlative cryo light microscopy and tomography/3D cryo volume imaging.

The new Crossbeam system features the Gemini 2 Electron column as well as the new Ion-sculptor FIB column. By making use of the FIB's improved low voltage performance at below 5kV, the ZEISS Crossbeam 550 is also able to generate RT TEM lamellae of less than 50nm by keeping amorphisation damage to a minimum.  A Kleindiek manipulator supports both RT and cryo lamella lift out solutions.

The ZEISS Crossbeam 550 FIB-SEM will be available for booking in late 2020.

Please contact CAM if you have any questions regarding this instrument.

Updated:  25 November 2020/Responsible Officer:  Director CAM/Page Contact:  CAM Web Admin