CAM is presenting a seminar by Dr Tetsuo Oikawa, Senior Project Manager for TEM for JEOL Ltd, Tokyo, Japan.

Featuring the unique combination of high resolution electron optics and patented in-column energy filter, JEOL TEM have taken imaging of biological samples at ambient and Cryo temperatures a step further.

For TEM imaging of biological systems Cryo temperatures have been used to reduce beam damage. In addition, Defocus Phase Contrast (DPC) has been used to improve image contrast.

New methods of contrast enhancement have been developed and tested as the result of attempts to use conditions for more efficient imaging in Cryo EM. Those are based on devices that filter inelastically scattered electrons and/or modulate the electron wave and change its phase characteristics resulting in big improvements in contrast and visibility of various features of the specimen.

The latest application data obtained using JEOL Omega filter and different types of phase plates installed in JEOL Cryo FE TEM will be presented and discussed.

Dr Oikawa graduated from Kogakuin University (Japan) in March 1980 and the same year joined JEOL Ltd, in charged of development of analytical TEM. Dr Oikawa's specialities include electron microscopy, analytical electron microscopy, electron optics and imaging.

2011 - present: TEM Application Specialist, JEOL Ltd.

2008 – 2011: Invited Professor of the University of Stockholm

2002 – 2012: Adjunct Professor at the University of Sydney

2007 – 2011: Director, European Application Group, JEOL (Europe)

2005: Assistant General Manager of the Electron Optics Division, JEOL

From 2005: Invited Professor of the University Paris 7

From 1994: Lecturer at Tohoku University

Seminar: Pushing Boundaries and Limiting Factors in Biological Cryo-EM (PDF)