Introduction to Light Microscopy

This workshop will provide participants with the knowledge and skill to:

  • set up and use basic light microscopes
  • collect accurate and meaningful digital images
  • be aware of technique limitations, thus enabling you to obtain the best image possible.

Main topics covered:

  • Overview of basic light microscopes, including optics and light paths
  • What all those numbers on the objective mean and why they are important
  • How to set up your microscope, including Köhler illumination
  • Introduction to resolution limits, and how to calculate them
  • Introduction to digital microscopy cameras
  • Sampling theory and how it all applies to image collection
  • Digital imaging artefacts, (and how to avoid them)
  • The complications of colour
  • Signal to noise, and sources of noise
  • Data formats, and
  • Digital imaging ethics.


CAM: Light Microscopes


ANU students: AUD$150.00 per person (ex-GST)

Non-ANU students: AUD$275.00 per person ($250 + GST)

Please contact CAM to register for this workshop.

Maximum number of students: 



 Josie Smith
 02 6125 3543
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Updated:  17 January 2021/Responsible Officer:  Director CAM/Page Contact:  CAM Web Admin