Introduction to Transmission Electron Microscopy (Life Sciences)

This course will provide participants with the knowledge and skill to:

  • set up and use transmission electron microscopes for imaging biological specimens
  • collect accurate and meaningful digital images, and
  • view different sample preparation techniques for TEM analysis.

Main topics covered

  • History of Transmission Electron Microscopy (TEM)
  • What is TEM?
  • Basic Components
  • The electron source
  • The electromagnetic lenses
  • The vacuum system
  • Magnification and Resolution
  • Image formation
  • Astigmatism
  • Bright vs. Dark Field Imaging
  • Biological specimen preparation
  • Imaging capabilities of a TEM
  • Artefacts
  • Optimising the TEM for biological imaging.


ANU students: AUD$150.00 per person.

Non-ANU students: AUD$275.00 per person ($250 + GST).

Hitachi H7100FA 125kV Transmission Electron Microscope


Maximum number of students: 



 Josie Smith
 +61 2 6125 3543
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Updated:  17 January 2021/Responsible Officer:  Director CAM/Page Contact:  CAM Web Admin