This workshop will provide participants with the knowledge and skill to:
- set up and use basic light microscopes
- collect accurate and meaningful digital images
- be aware of technique limitations, thus enabling you to obtain the best image possible.
Main topics covered
- Overview of basic light microscopes, including optics and light paths
- What all those numbers on the objective mean and why they are important
- How to set up your microscope, including Köhler illumination
- Introduction to resolution limits, and how to calculate them
- Introduction to digital microscopy cameras
- Sampling theory and how it all applies to image collection
- Digital imaging artefacts, (and how to avoid them)
- The complications of colour
- Signal to noise, and sources of noise
- Data formats, and
- Digital imaging ethics.
Please contact CAM to register for this workshop.
Maximum number of students: 8