Dr Jobichen Chacko

CRYO EM Specialist

Jobichen is a structural biologist with extensive experience in protein structural biology, protein-ligand interaction, proteases and drug discovery. He completed his PhD from National University of Singapore in the area of structural and functional studies on bacterial protein secretion system. He is proficient in the areas of Cryo-Electron microscopy (Single Particle Analysis), protein structure analysis and X-ray crystallography. He has published more than 35 research articles in high level journals and has more than 400 citations. His research interests are in host-pathogen interaction, protein secretion system, snake venom toxins and proteases. Jobichen is a member of the editorial advisory board for FEBS-openbio journal and serves as a reviewer for various scientific journals.

Research interests

Jobichen's main work is in the areas of structural biology with particular interest in Cryo-Electron microscopy. His research interests lie mainly in the areas of host-pathogen interaction and proteases. He is also working on various projects related to snake venom proteins and bacterial secretion system proteins.

Selected publications:

Varsha AW, Karthik R, Jobichen C, Muthu K, J. Sivaraman, Kini RM, MokYK. 2021. Crystal structure of Aedes aegypti trypsin inhibitor in complex with μ-plasmin reveals role for scaffold stability in Kazal-type serine protease inhibitor. Protein Science. 2021 November 18

Chichili VP, Chew TW, Shankar S, Er SY, Chin FC, Jobichen C, et al; 2021.  Structural basis for p50RhoGAP BCH domain–mediated regulation of Rho inactivation. PNAS: 118 e2014242118.

Gavor E, Choong YK, Jobichen C, Mok YK, Kini RM, Sivaraman J. 2021. Structure of Aedes aegypti carboxypeptidase B1‐inhibitor complex uncover the disparity between mosquito and non‐mosquito insect carboxypeptidase inhibition Protein Science. 2021 Oct 18.

Jobichen C, Chong TY, Hui L and J Sivaraman. 2021. Autocatalytic cleavage domain is not required for the activity of ScpC, a virulence protease from Streptococcus pyogenes: A structural insight. Biochemistry. 60(19):1564-1568

Jingru Chan, et al, Jobichen C. 2021. Structural and mechanistic insight into stem-loop RNA processing by yeast Pichia stipitis Dicer. Protein Science: 30(6):1210-1220.

Jobichen C, Chong TY, et al, 2021. Cryo-EM Structure of bacterioferritin nanocages provides insight into the bio-mineralization of ferritins. bioRxiv: https://doi.org/10.1101/2021.02.04.429857

Jobichen C, Prabhakar, M; Loh, S N; Sivaraman, J. 2020. Structural basis for the unique inhibitory mechanism of ecotin against neutrophil elastase. Biochemistry: 59(30): 2788-95

Ying Chen, Jingru Chan, et al, Jobichen C.2020. SYNCRIP, a new player in pri-let-7a processing.  RNA. 26(3): 290-305.

Narumi A-S, Jobichen C, et al (2020). Unusual quaternary structure of a homodimeric synergistic toxin from mamba snake venom. Biochem J. 477(20):3951-3962

Foo CS, Jobichen C, et al; 2020. Fulditoxin, representing a new class of dimeric snake toxins, defines novel pharmacology at nicotinic acetylcholine receptors. Br J Pharmacol. 177(8):1822-1840

Singh AK, Datta A, Jobichen C, Luan S, Vasudevan D. 2020. AtFKBP53: a chimeric histone chaperone with functional nucleoplasmin and PPIase domains. Nucleic Acids Res. 48(3):1531-1550

Roy A, Sun Q, Alex C, Rajagopalan N, Jobichen C, et al 2019. Identification of a α-helical molten globule intermediate and structural characterization of β-cardiotoxin, an all β-sheet protein isolated from the venom of Ophiophagus hannah (King cobra). Protein Science. 28(5): 952-963

Bee HL, Jobichen C, et al 2018. Targeting cancer addiction for SALL4 by shifting its transcriptome with a pharmacologic peptide. PNAS: 115(30) E7119-E7128                

Noreen Z, Jobichen C, et al 2018. Structural basis for the pathogenesis of Campylobacter jejuni Hcp1, a structural and effector protein of the type six secretion system. FEBS Journal. 285(21): 4060-4070.

Umar FSH, Oana S, Sui TL, Sarah JA, Jobichen C, et al 2018. Proteobacterial origin of protein arginine methylation and regulation of complex I assembly by MidA. Cell Reports: 21; 24(8):1996-2004.