Introduction to Light Microscopy

This workshop will provide participants with the knowledge and skill to:

  • set up and use basic light microscopes
  • collect accurate and meaningful digital images
  • be aware of technique limitations, thus enabling you to obtain the best image possible.

Main topics covered

  • Overview of basic light microscopes, including optics and light paths
  • What all those numbers on the objective mean and why they are important
  • How to set up your microscope, including Köhler illumination
  • Introduction to resolution limits, and how to calculate them
  • Introduction to digital microscopy cameras
  • Sampling theory and how it all applies to image collection
  • Digital imaging artefacts, (and how to avoid them)
  • The complications of colour
  • Signal to noise, and sources of noise
  • Data formats, and
  • Digital imaging ethics.

Please contact CAM to register for this workshop.

Maximum number of students: 8