This workshop will provide participants with the knowledge and skill to:

• set up and use basic light microscopes
• collect accurate and meaningful digital images
• be aware of technique limitations, thus enabling you to obtain the best image possible.

Main topics covered

• Overview of basic light microscopes, including optics and light paths
• What all those numbers on the objective mean and why they are important
• How to set up your microscope, including Köhler illumination
• Introduction to resolution limits, and how to calculate them
• Introduction to digital microscopy cameras
• Sampling theory and how it all applies to image collection
• Digital imaging artefacts, (and how to avoid them)
• The complications of colour
• Signal to noise, and sources of noise
• Data formats, and
• Digital imaging ethics.

Please contact CAM to register for this workshop.

Maximum number of students: 8