Introduction to Transmission Electron Microscopy (Life Sciences)

This course will provide participants with the knowledge and skill to:

  • set up and use transmission electron microscopes for imaging biological specimens
  • collect accurate and meaningful digital images, and
  • view different sample preparation techniques for TEM analysis.

Main topics covered

  • History of Transmission Electron Microscopy (TEM)
  • What is TEM?
  • Basic Components
  • The electron source
  • The electromagnetic lenses
  • The vacuum system
  • Magnification and Resolution
  • Image formation
  • Astigmatism
  • Bright vs. Dark Field Imaging
  • Biological specimen preparation
  • Imaging capabilities of a TEM
  • Artefacts
  • Optimising the TEM for biological imaging.

Hitachi H7100FA 125kV Transmission Electron Microscope

Please register your interest for the next workshop here.

Maximum number of students: 8