ZEISS Crossbeam 550 FIB-SEM

ZEISS Crossbeam 550 FIB/FESEM An electron microscope sits on the floor of a room, to the right of a table that has two computer monitors with a keyboard and other controls on it. There is an armless office chair in front of the table.

For Life Sciences research

The Crossbeam comprises a Leica cryo stage and transfer system which will facilitate cryo block face imaging, as well as the production of TEM lamella from cells or tissues for viewing in the JEOL 200kV CryoARM (NCRIS co-funded).

For a correlative workflow, additional upgrades include a new Linkam Scientific cryo stage (CMS196) for our confocal LM (ZEISS LSM800 with Airyscan) and the ZEISS Atlas 5 and ZEN Connect software, which will facilitate an enhanced workflow for correlative cryo light microscopy and tomography/3D cryo volume imaging.

For Materials Sciences research

The new Crossbeam system features the Gemini 2 Electron column as well as the new Ion-sculptor FIB column.

By making use of the FIB's improved low voltage performance at below 5kV, the ZEISS Crossbeam 550 is also able to generate TEM lamellae (at cryo or room temperature) of less than 50nm by keeping amorphisation damage to a minimum.  A Kleindiek manipulator supports both RT and cryo lamella lift out solutions.