Advanced Topics in Light Microscopy

Advanced Topics in Light Microscopy
Summary
The "Advanced Topics in Light Microscopy" course at ANU's Centre for Advanced Microscopy is a two-day program designed to enhance participants' skills in advanced microscopy techniques, including Phase contrast, Interference contrast, Polarising, and Fluorescent microscopy.
Course type
Course type
Professional short course
Contact
Contact
This is a two-day course, that builds on the introductory course and will provide students with the knowledge required to:
- set up and use Phase contrast, Interference contrast and Polarising microscopes
- select appropriate dyes and filter sets for Fluorescent microscopy
- choose appropriate Fluorescent imaging modalities
- be critical of data and troubleshoot their imaging.
Topics covered include, but not limited to:
- Components and light paths in Phase contrast, Interference contrast and Polarising microscopes, including reflected light techniques
- Microscope Beam Paths and Components in fluorescent microscopy
- Properties of Fluorescence molecules and Fluorophore selection
- The emission equation
- Properties of filters and the filter cube
- Matching filters and fluorochromes
- Fluorescent antibody and protein experimental design considerations
- Principles, characteristics and the pros and cons of Widefield Fluorescence, Deconvolution Microscopy Structured illumination (Apotome), Confocal and Multiphoton Microscopy (When to use what!)
- Identifying problems and finding solutions
- Resolution and Signal to Noise issues
- Optical aberrations: what are they, what do they do to our image and how do we fix it?
- Introduction to the principles behind Spectral unmixing,TIRF, Super Resolution, STED, PALM, STORM, SIM GSD, and their relatives
- Non-imaging fluorescence techniques: FCS, FLIM.
Cost: AUD$250.00 per person (GST not included)
Please note: this workshop is offered on demand.
Presenters
Cost
AUD$250.00 per person (GST not included)