This is a two-day course, that builds on the introductory course and will provide students with the knowledge required to:

• set up and use Phase contrast, Interference contrast and Polarising microscopes
• select appropriate dyes and filter sets for Fluorescent microscopy
• choose appropriate Fluorescent imaging modalities
• be critical of data and troubleshoot their imaging.

Topics covered include, but not limited to:

• Components and light paths in Phase contrast, Interference contrast and Polarising microscopes, including reflected light techniques
• Microscope Beam Paths and Components in fluorescent microscopy
• Properties of Fluorescence molecules and Fluorophore selection
• The emission equation
• Properties of filters and the filter cube
• Matching filters and fluorochromes
• Fluorescent antibody and protein experimental design considerations
• Principles, characteristics and the pros and cons of Widefield Fluorescence, Deconvolution Microscopy Structured illumination (Apotome), Confocal and Multiphoton Microscopy (When to use what!)
• Identifying problems and finding solutions
• Resolution and Signal to Noise issues
• Optical aberrations: what are they, what do they do to our image and how do we fix it?
• Introduction to the principles behind Spectral unmixing,TIRF, Super Resolution, STED, PALM, STORM, SIM GSD, and their relatives
• Non-imaging fluorescence techniques: FCS, FLIM.

Cost: AUD$250.00 per person (GST not included)

Please note: this workshop is offered on demand.